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Detection of Weakly Expressed Trypanosoma cruzi Membrane Proteins Using High‐Performance Probes
Author(s) -
CruzBustos Teresa,
Moreno Silvia N.J.,
Docampo Roberto
Publication year - 2018
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/jeu.12517
Subject(s) - trypanosoma cruzi , biology , subcellular localization , microbiology and biotechnology , membrane protein , epitope , crispr , membrane , computational biology , biochemistry , gene , antibody , genetics , cytoplasm , parasite hosting , world wide web , computer science
Membrane proteins in trypanosomatids are, in general, weakly expressed and confirmation of their subcellular localization frequently requires their overexpression with epitope tags. However, overexpression can lead to mislocalization of the probes. Viswanathan et al. ( Nat. Methods , 2015, 12:568) described high performance tags for localization of weakly expressed proteins. We report here the use of these protein tags, named “spaghetti monster,” for CRISPR /Cas9‐mediated C‐terminal endogenous tagging of Trypanosoma cruzi to localize two weakly expressed transient receptor potential channels to acidic compartments. The results indicate that this method will improve the detection of membrane proteins in T. cruzi .