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Replication Protein A‐1 Has a Preference for the Telomeric G‐rich Sequence in Trypanosoma cruzi
Author(s) -
Pavani Raphael Souza,
Vitarelli Marcela O.,
Fernandes Carlos A. H.,
Mattioli Fabio F.,
Morone Mariana,
Menezes Milene C.,
Fontes Marcos R. M.,
Cano Maria Isabel N.,
Elias Maria Carolina
Publication year - 2017
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/jeu.12478
Subject(s) - biology , replication protein a , telomere , dna replication , trypanosoma cruzi , dna damage , dna , telomerase , microbiology and biotechnology , dna repair , heterotrimeric g protein , viral replication , dna binding protein , genetics , signal transduction , gene , cell culture , parasite hosting , world wide web , transcription factor , computer science , g protein
Replication protein A ( RPA ), the major eukaryotic single‐stranded binding protein, is a heterotrimeric complex formed by RPA ‐1, RPA ‐2, and RPA ‐3. RPA is a fundamental player in replication, repair, recombination, and checkpoint signaling. In addition, increasing evidences have been adding functions to RPA in telomere maintenance, such as interaction with telomerase to facilitate its activity and also involvement in telomere capping in some conditions. Trypanosoma cruzi , the etiological agent of Chagas disease is a protozoa parasite that appears early in the evolution of eukaryotes. Recently, we have showed that T. cruzi RPA presents canonical functions being involved with DNA replication and DNA damage response. Here, we found by FISH / IF assays that T. cruzi RPA localizes at telomeres even outside replication (S) phase. In vitro analysis showed that one telomeric repeat is sufficient to bind RPA ‐1. Telomeric DNA induces different secondary structural modifications on RPA ‐1 in comparison with other types of DNA . In addition, RPA ‐1 presents a higher affinity for telomeric sequence compared to randomic sequence, suggesting that RPA may play specific roles in T. cruzi telomeric region.