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Tf VPS 32 Regulates Cell Division in the Parasite Tritrichomonas foetus
Author(s) -
Iriarte Lucrecia S.,
Midlej Victor,
Frontera Lorena S.,
Moros Duarte Daniel,
Barbeito Claudio G.,
Souza Wanderley,
Benchimol Marlene,
Miguel Natalia,
Coceres Veronica M.
Publication year - 2017
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/jeu.12424
Subject(s) - biology , tritrichomonas foetus , microbiology and biotechnology , cell division , cell cycle , multinucleate , cytoplasm , mitosis , cytokinesis , midbody , cell , fetus , genetics , pregnancy
The flagellated protist Tritrichomonas foetus is a parasite that causes bovine trichomonosis, a major sexually transmitted disease in cattle. Cell division has been described as a key player in controlling cell survival in other cells, including parasites but there is no information on the regulation of this process in T. foetus . The regulation of cytokinetic abscission, the final stage of cell division, is mediated by members of the ESCRT (endosomal sorting complex required for transport) machinery. VPS 32 is a subunit within the ESCRTIII complex and here, we report that Tf VPS 32 is localized on cytoplasmic vesicles and a redistribution of the protein to the midbody is observed during the cellular division. In concordance with its localization, deletion of Tf VPS 32 C‐terminal alpha helices (α5 helix and/or α4‐5 helix) leads to abnormal T. foetus growth, an increase in the percentage of multinucleated parasites and cell cycle arrest at G2/M phase. Together, these results indicate a role of this protein in controlling normal cell division.