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Comparison of Recombinant Proteins of Kinesin 39, Heat Shock Protein 70, Heat Shock Protein 83, and Glycoprotein 63 for Antibody Detection of Leishmania martiniquensis Infection
Author(s) -
Siripattanapipong Suradej,
Kato Hirotomo,
Tanariya Peerapan,
Mungthin Mathirut,
Leelayoova Saovanee
Publication year - 2017
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/jeu.12415
Subject(s) - biology , visceral leishmaniasis , virology , antigen , antibody , recombinant dna , leishmania infantum , heat shock protein , serology , dipstick , epitope , western blot , leishmania donovani , leishmania , leishmaniasis , immunology , parasite hosting , biochemistry , urine , world wide web , computer science , gene
Leishmania martiniquensis , a zoonotic hemoflagellate, is a causative agent of cutaneous ( CL ) and visceral leishmaniasis ( VL ) among humans and animals. This organism, first reported in Martinique Island, now has become an emerging infectious agent in Thailand. Symptomatic cases of L . martiniquensis infection among humans have continuously increased. In the meantime, asymptomatic infection of this novel species has seriously created national public health awareness and concern to prevent and control disease transmission. The unsuccessful serological test using the commercial rK 39 dipstick based on antigen from Leishmania donovani to detect the antibodies against VL among infected Thai patients has encouraged us to further explore a new sensitive and specific antigenic epitope. In this study, we determined the sequences and expressed recombinant proteins of kinesin 39 ( k 39), heat shock protein 70 ( hsp 70), heat shock protein 83 ( hsp 83), and glycoprotein 63 ( gp 63) of L . martiniquensis to evaluate the diagnostic efficiency to detect antibodies against L. martiniquensis in patient sera. The preliminary results from western blot analysis have suggested that K39 is the most sensitive recombinant protein to detect L . martiniquensis . Moreover, this recombinant protein reacts with antibodies against L. donovani and Leishmania infantum , making it a promising antigen for further development of a universal rapid diagnostic tool for VL .