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Bacterium‐Expressed ds RNA Downregulates Microsporidia Nosema bombycis Gene Expression
Author(s) -
Pan Qiuling,
Wang Ling,
Dang Xiaoqun,
Ma Zhengang,
Zhang Xiaoyan,
Chen Shiliang,
Zhou Zeyang,
Xu Jinshan
Publication year - 2016
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/jeu.12346
Subject(s) - biology , microsporidia , nosema , rna silencing , gene , rna , pathogen , gene expression , rna interference , virology , microbiology and biotechnology , genetics , spore
The microsporidia Nosema bombycis is the insect pathogen of pebrine disease severely destructive to sericulture production. Here, we describe the use of Escherichia coli HT 115 strain ( DE 3) to express double‐strand RNA s targeting the gene encoding ADP / ATP protein in N. bombycis . The results showed that ds RNA s deferentially suppressed the gene expression during N. bombycis infection in the silkworm, and the effect waned gradually. Our results, for the first time, provide a tool to utilize the ds RNA expressed by recombinant E. coli to control the pebrine disease of the domestic silkworm.