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Genotypes of Cryptosporidium spp. and Enterocytozoon bieneusi in Human Immunodeficiency Virus‐Infected Patients in Lagos, Nigeria
Author(s) -
Ojuromi Oladele T.,
Duan Liping,
Izquierdo Fernando,
Fenoy Soledad M.,
Oyibo Wellington A.,
Aguila Carmen,
Ashafa Anofi O. T.,
Feng Yaoyu,
Xiao Lihua
Publication year - 2015
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/jeu.12285
Subject(s) - enterocytozoon bieneusi , biology , cryptosporidium , virology , genotype , microsporidiosis , human immunodeficiency virus (hiv) , microbiology and biotechnology , microsporidia , feces , genetics , gene , spore
Molecular characterization of Cryptosporidium spp. and Enterocytozoon bieneusi has improved our understanding of the transmission of both organisms in humans. In this study, to infer possible infection sources, Cryptosporidium spp. and E. bieneusi in fecal specimens from 90 HIV ‐infected patients attending antiretroviral clinics in Lagos, Nigeria were detected and genotyped by PCR and DNA sequencing. Cryptosporidium spp. and E. bieneusi were identified in four and five patients, respectively, including the occurrence of subtype IeA11T3G3 of Cryptosporidium hominis in two patients, subtype II cA5G3k of Cryptosporidium parvum in one patient, and Type IV of E. bieneusi in four patients. Among the remaining positive patients, one had mixed infection of Cryptosporidium meleagridis and C. hominis and one had mixed E. bieneusi genotypes. These data highlight a possible difference in major transmission routes (anthroponotic vs. zoonotic) between Cryptosporidium spp. and E. bieneusi in HIV + patients in the study area.