Premium
High Prevalence and Widespread Distribution of Zoonotic Enterocytozoon bieneusi Genotypes in Swine in Northeast China: Implications for Public Health
Author(s) -
Wan Qiang,
Lin Yongchao,
Mao Yixian,
Yang Yuqi,
Li Qiao,
Zhang Siwen,
Jiang Yanxue,
Tao Wei,
Li Wei
Publication year - 2015
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/jeu.12264
Subject(s) - enterocytozoon bieneusi , microsporidiosis , biology , genotype , internal transcribed spacer , feces , veterinary medicine , zoonosis , cryptosporidium , ribosomal rna , nested polymerase chain reaction , polymerase chain reaction , virology , microbiology and biotechnology , microsporidia , gene , genetics , medicine , spore
This study analyzed 563 fecal specimens of asymptomatic pigs from five cities of northeast China for the prevalence and genetic characteristics of Enterocytozoon bieneusi . The parasite was detected in 267 of 563 (47.4%) pigs by nested PCR of the ribosomal internal transcribed spacer ( ITS ). The differences in prevalence between preweaned (58.0%, 94/162) and growing pigs (39.6%, 114/288) and between weaned (52.2%, 59/113) and growing pigs are significant ( p < 0.05). Genotypic typing and phylogenetic analysis facilitated identification of six human‐pathogenic genotypes EbpC, O, CS ‐4, EbpA, Henan‐ IV , and Pig EBITS 5 and six potentially zoonotic genotypes EbpB, CC ‐1, CS ‐1, CS ‐3, CHN 7, and CS ‐10. Genotypes CS ‐4 (32/35) and EbpC (3/35) from Harbin and Henan‐ IV (5/64) from Qiqihar determined in pigs herein represented the main causative agents of human microsporidiosis in Harbin. The most dominant genotype EbpC found in pigs from Daqing (35/65) and Qiqihar (a close neighbor to Daqing) (47/64) contributed significantly to human infections in Daqing. Genotype EbpC was also a leading E. bieneusi pathogen in humans, drinking water, and wastewater in central China. This study provided robust evidence that pigs could be an outstanding source of human microsporidiosis and water contamination in China.