Premium
Fast Technology Analysis Enables Identification of Species and Genotypes of Latent Microsporidia Infections in Healthy Native Cameroonians
Author(s) -
Ndzi Edward S.,
Asonganyi Tazoacha,
Nkinin Mary Bello,
Xiao Lihua,
Didier Elizabeth S.,
Bowers Lisa C.,
Nkinin Stephenson W.,
Kaneshiro Edna S.
Publication year - 2015
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/jeu.12262
Subject(s) - microsporidia , biology , enterocytozoon bieneusi , encephalitozoon cuniculi , microsporidiosis , amplicon , genotype , digital polymerase chain reaction , feces , zoology , virology , polymerase chain reaction , microbiology and biotechnology , genetics , spore , gene
Several enteric microsporidia species have been detected in humans and other vertebrates and their identifications at the genotype level are currently being elucidated. As advanced methods, reagents, and disposal kits for detecting and identifying pathogens become commercially available, it is important to test them in settings other than in laboratories with “state‐of‐the‐art” equipment and well‐trained staff members. In the present study, we sought to detect microsporidia DNA preserved and extracted from FTA (fast technology analysis) cards spotted with human fecal suspensions obtained from Cameroonian volunteers living in the capital city of Yaoundé to preclude the need for employing spore‐concentrating protocols. Further, we tested whether amplicon nucleotide sequencing approaches could be used on small aliquots taken from the cards to elucidate the diversity of microsporidia species and strains infecting native residents. Of 196 samples analyzed, 12 (6.1%) were positive for microsporidia DNA ; Enterocytozoon bieneusi (Type IV and KIN ‐1), Encephalitozoon cuniculi , and Encephalitozoon intestinalis were identified. These data demonstrate the utility of the FTA cards in identifying genotypes of microsporidia DNA in human fecal samples that may be applied to field testing for prevalence studies.