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A Molecular Phylogenetic Investigation of Bakuella , Anteholosticha, and Caudiholosticha (Protista, Ciliophora, Hypotrichia) Based on Three‐Gene Sequences
Author(s) -
Lv Zhao,
Shao Chen,
Yi Zhenzhen,
Warren Alan
Publication year - 2014
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/jeu.12194
Subject(s) - biology , phylogenetic tree , monophyly , phylogenetics , taxon , evolutionary biology , molecular phylogenetics , zoology , genetics , gene , clade , ecology
Traditionally classifications of the Urostyloida have been mainly based on morphology and morphogenesis. Recent molecular phylogenetic analyses have been largely based on single‐gene data for a limited number of taxa. Consequently, incongruence has arisen between the morphological/morphogenetic and the molecular data. In this study, the three phylogenetic markers (SSU rDNA, ITS1‐5.8S‐ITS2 region, and LSU‐rDNA) of three urostyloid genera represented by four species ( Bakuella granulifera , Anteholosticha monilata , Caudiholosticha sylvatica, and C. tetracirra ) were sequenced to investigate their phylogeny. The results show that: (1) all three genera should be regarded as the members of the order Urostyloida within the subclass Hypotrichia, as indicated by morphological characters; (2) phylogenetic analyses and sequence similarities both indicate that neither Anteholosticha nor Caudiholosticha are monophyletic and the systematic assignment of both genera awaits further evaluation; and (3) Bakuella has a closer relationship with Urostyla than with bakuellids (e.g. Apobakuella and Metaurostylopsis ), suggesting Bakuella may belong to the family Urostylidae rather than the family Bakuellidae.