Toxoplasma gondii  Isolates from Mouflon Sheep ( Ovis ammon ) from Hawaii,  USA | Zendy

Verma Shiv K. | Zendy; Su Chunlei | Zendy; Dubey Jitender P. | Zendy

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Toxoplasma gondii Isolates from Mouflon Sheep ( Ovis ammon ) from Hawaii, USA

Author(s) -

Verma Shiv K.,

Su Chunlei,

Dubey Jitender P.

Publication year - 2014

Publication title -

journal of eukaryotic microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.067

H-Index - 77

eISSN - 1550-7408

pISSN - 1066-5234

DOI - 10.1111/jeu.12151

Subject(s) - biology , toxoplasma gondii , ovis , genotyping , genotype , restriction fragment length polymorphism , virology , veterinary medicine , genetics , antibody , ecology , gene , medicine

Little is known of Toxoplasma gondii isolates circulating in wildlife. The mouflon ( Ovis ammon ) is very popular game animal, hunted for its trophy horns. Here, we report the isolation and genetic characterization of T. gondii from two mouflons from Hawaii, USA . Both sheep had antibodies titers of 1:800 or higher. Viable T. gondii were isolated and nested PCR ‐ RFLP genotyping revealed two genotypes, a clonal Type III (designated TgMouflon US 1), and a new genotype (designated TgMouflon US 2, and Toxo DB PCR ‐ RFLP genotypes #249). This is the first report of T. gondii infection, isolation and genetic characterization in mouflons from the USA .

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