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Enterocytozoon bieneusi Genotypes in Yaks ( Bos grunniens ) and Their Public Health Potential
Author(s) -
Ma Jingbo,
Cai Jinzhong,
Ma Jiawen,
Feng Yaoyu,
Xiao Lihua
Publication year - 2014
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/jeu.12141
Subject(s) - enterocytozoon bieneusi , biology , genotype , internal transcribed spacer , nested polymerase chain reaction , feces , microsporidiosis , polymerase chain reaction , ribosomal dna , ribosomal rna , virology , microbiology and biotechnology , veterinary medicine , genetics , phylogenetics , gene , microsporidia , spore , medicine
Enterocytozoon bieneusi , the most frequently diagnosed microsporidian species in humans, is also identified in a wide range of animals. To date, few data are available on E. bieneusi in yaks ( Bos grunniens ). In this study, we examined the occurrence and genotype identity of E. bieneusi in yaks in four counties in Qinghai Province of China. Of 327 fecal specimens examined by nested PCR analysis of the ribosomal internal transcribed spacer, 23 (7.0%) were E. bieneusi ‐positive. DNA sequence analysis of the PCR products revealed the presence of five distinct genotypes: three Group 2 genotypes previously reported in cattle as well as humans ( BEB 4, I and J) and two novel genotypes ( CHN 11 and CHN 12) belonging to the large zoonotic group (Group 1). Data of the study suggest that these animals could be potential reservoirs for human E. bieneusi infection.