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Morphological and Ribosomal DNA ‐based Characterization of Six Antarctic Ciliate Morphospecies from the Amundsen Sea with Phylogenetic Analyses
Author(s) -
Kim Sun Young,
Choi Joong Ki,
Dolan John R.,
Shin Hyoung Chul,
Lee SangHoon,
Yang Eun Jin
Publication year - 2013
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/jeu.12057
Subject(s) - biology , ciliate , phylogenetic tree , ribosomal dna , ribosomal rna , botany , phylogenetic relationship , zoology , evolutionary biology , genetics , gene
We characterized six tintinnid ciliates from Antarctic waters using molecular markers and morphological traits: Amphorellopsis quinquealata , Codonellopsis gaussi , Cymatocylis convallaria , Cy. calyciformis , Cy. drygalskii, and Laackmanniella prolongata . The 100% similarity in SSU‐ITS1‐5.8S rDNA ‐ITS2‐partial LSU rDNA sequences among Cy. convallaria , Cy. calyciformis, and Cy. drygalskii is supportive of synonymy. Codonellopsis gaussi and L. prolongata also showed high levels of similarity in SSU rDNA (99.83%) and the D2 domain of LSU rDNA (95.77%), suggesting that they are closely related. Phylogenetic analysis placed Cymatocylis in the Rhabdonellidae, Amphorellopsis in the Tintinnidae and L. prolongata/Co. gaussi within the Dictyocystidae.