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Heat Shock Protein 90 of B onamia ostreae : Characterization and Possible Correlation with Infection of the Flat O yster, O strea edulis
Author(s) -
PradoAlvarez Maria,
Chollet Bruno,
Couraleau Yann,
Morga Benjamin,
Arzul Isabelle
Publication year - 2013
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/jeu.12031
Subject(s) - biology , ostrea edulis , open reading frame , heat shock protein , complementary dna , hsp90 , cytosol , intracellular , parasite hosting , microbiology and biotechnology , oyster , gene , genetics , biochemistry , peptide sequence , fishery , enzyme , world wide web , computer science
In this study, we described the cytosolic HSP 90 of B onamia ostreae , an intracellular parasite of O strea edulis hemocytes. The complete open reading frame was assembled by Rapid Amplification cDNA Ends reactions on cDNA of B . ostreae ‐infected hemocytes. HSP 90 amplification was corroborated in infected oysters and B . ostreae purified cells. The functionality of the HSP 90, studied by inhibitory assays with radicicol, suggests that this protein may play a role in hemocyte invasion. Our results inform the molecular basis that governs B . ostreae– O . edulis interactions.

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