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Effect of Condensed Tannins on Bovine Rumen Protist Diversity Based on 18S rRNA Gene Sequences
Author(s) -
Tan Hui Yin,
Sieo Chin Chin,
Abdullah Norhani,
Liang Juan Boo,
Huang Xiao Dan,
Ho Yin Wan
Publication year - 2012
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/jeu.12011
Subject(s) - biology , protist , rumen , 18s ribosomal rna , ribosomal rna , gene , diversity (politics) , genetics , food science , fermentation , sociology , anthropology
Molecular diversity of protists from bovine rumen fluid incubated with condensed tannins of L eucaena leucocephala hybrid‐Rendang at 20 mg/500 mg dry matter (treatment) or without condensed tannins (control) was investigated using 18S rRNA gene library. Clones from the control library were distributed within nine genera, but clones from the condensed tannin treatment clone library were related to only six genera. Diversity estimators such as abundance‐based coverage estimation and Chao1 showed significant differences between the two libraries, although no differences were found based on S hannon– W eaver index and L ibshuff.