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P hytophthora infestans Field Isolates from G ansu P rovince, C hina are Genetically Highly Diverse and Show a High Frequency of Self Fertility
Author(s) -
Han Miao,
Liu Gang,
Li JiPing,
Govers Francine,
Zhu XiaoQiong,
Shen ChongYao,
Guo LiYun
Publication year - 2012
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/jeu.12010
Subject(s) - biology , mating type , phytophthora infestans , genotype , genetic diversity , haplotype , analysis of molecular variance , sexual reproduction , genotyping , population , genetics , veterinary medicine , gene , medicine , demography , sociology
The genetic diversity of 85 isolates of P hytophthora infestans collected in 2007 from G ansu province in C hina was determined and compared with 21 isolates collected before 2004. Among them, 70 belonged to the A1 mating type and 15 were self‐fertile ( SF ). The mitochondrial DNA haplotypes revealed both Ia (25%) and IIa (75%) haplotypes. Metalaxyl resistance occurred with high frequency (54%) in G ansu. Simple sequence repeat ( SSR ) genotyping revealed 26 genotypes (13 from the T ianshui region) among the 85 isolates, and 18 genotypes among the 21 isolates collected before 2004, without overlap in genotypes detected in the two groups. Cluster analysis showed clear subdivisions within the different mating type isolates. Among G ansu's isolates, N ei's and S hannon's diversity indices were highest in isolates collected in T ianshui where both A1 and SF isolates were found. Analysis of molecular variance of isolates from G ansu indicated that 51% and 49% of the variance was explained by within‐area and among‐area variance, respectively. The results suggest that the occurrence of SF isolates increases the risk of sexual reproduction, the formation of oospore as initial inocula in the field, and affects the genotypic diversity in the population.