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Effect of acaricidal compound extracted from Arachis hypogaea Linn against Tetranychus cinnabarinus
Author(s) -
Liu Yaya,
Liu Jia,
Gao Yanjin,
Yao Jianhua,
Zhao Jie,
Dai Guanghui
Publication year - 2019
Publication title -
journal of applied entomology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 60
eISSN - 1439-0418
pISSN - 0931-2048
DOI - 10.1111/jen.12654
Subject(s) - arachis hypogaea , petroleum ether , acaricide , palmitic acid , chromatography , biology , ethyl acetate , distilled water , extraction (chemistry) , bioassay , silica gel , ether , botany , toxicology , chemistry , organic chemistry , biochemistry , fatty acid , genetics
Six plant crude extracts were chosen to evaluate their acaricidal activity against Tetranychus cinnabarinus adults. The crude extract from the stems and leaves of Arachis hypogaea L. presented the highest activity in leaf‐dip bioassays, with an LC 50 value of 3,545.98 mg/L. Further extraction using four different solvents (petroleum ether, ethyl acetate, n‐butyl alcohol and distilled water) demonstrated that the active components mainly existed in the petroleum ether phase. Then, the active compound, palmitic acid, was isolated from the petroleum ether phase via two‐step column chromatography using columns filled with silica gel and C‐18 and identified via mass spectrometry and nuclear magnetic resonance analyses. The LC 50 value of active palmitic acid against T. cinnabarinus was 534.58 mg/L. The present study demonstrated that the active compound extracted from A. hypogaea is a potential novel botanical acaricide for controlling T. cinnabarinus .