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Expression pattern and ligand‐binding properties of odorant‐binding protein 13 from Monochamus alternatus hope
Author(s) -
Li N.,
Sun X.,
Wang M.Q.
Publication year - 2017
Publication title -
journal of applied entomology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 60
eISSN - 1439-0418
pISSN - 0931-2048
DOI - 10.1111/jen.12396
Subject(s) - biology , ligand binding assay , odorant binding protein , recombinant dna , ligand (biochemistry) , biochemistry , microbiology and biotechnology , botany , receptor , gene
Odorant‐binding proteins ( OBP s) are believed to play an important role in olfactory recognition. In this study, expression pattern and fluorescence binding characteristics of Malt OBP 13 from the Japanese pine sawyer beetle, Monochamus alternatus Hope, were investigated via qPCR analysis of Malt OBP 13 mRNA level and binding assay of Malt OBP 13 and ligands. qPCR monitoring indicated Malt OBP 13 mainly expressed in newly emerged males, particularly highly expressed in the last abdominal segment of males, and the expression level was significantly higher in 13‐day‐old mated adults than those of other stages. To further understand the function of the Malt OBP 13 protein in odorant reception, the binding affinity of recombinant Malt OBP 13 to ligands was tested by fluorescence binding assays with N ‐phenyl‐1‐naphthylamine as a fluorescent probe. The results of this assay indicated that Malt OBP 13 exhibited a high binding affinity for pine volatiles and binding capacity was higher in acidic conditions than in neutral environment, indicating a possible role in finding host plants.