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New Bacillus thuringiensis strain isolated from the gut of Malabari goat is effective against Tetranychus macfarlanei
Author(s) -
Neethu K. B.,
Priji P.,
Unni K. N.,
Sajith S.,
Sreedevi S.,
Ramani N.,
Anitha K.,
Rosana B.,
Girish M. B.,
Benjamin S.
Publication year - 2016
Publication title -
journal of applied entomology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 60
eISSN - 1439-0418
pISSN - 0931-2048
DOI - 10.1111/jen.12235
Subject(s) - bacillus thuringiensis , biology , maltose , pellet , sucrose , spore , lactose , bioassay , sugar , food science , husk , zoology , botany , veterinary medicine , microbiology and biotechnology , bacteria , genetics , medicine
This study illustrates a novel strain (designated as BPU 5) of Bacillus thuringiensis ( Bt ) isolated from the rumen of Malabari goat, capable of producing polymorphic δ ‐endotoxin crystals concomitantly with sporulation in Luria–Bertani medium ( LB ), and the δ ‐endotoxin was efficient to combat Tetranychus macfarlanei, a devastating mite. Polymorphic δ ‐endotoxin crystals produced were assessed by scanning electron microscopy and monitored its production concomitantly with sporulation in LB with or without sugar supplements. Toxicity of the δ ‐endotoxin was assessed on T. macfarlanei using leaf disc bioassay method. Mortality rate was determined by comparing the survival of mites on the diet (prepared in 10% sucrose and powdered rice husk) containing different concentrations (1–10 mg/ml) of 72‐h‐old crude pellet (dried mixture of δ ‐endotoxin (17 mg/g pellet), endospores and a few vegetative cells) or control diet with autoclaved pellet. The maximum production (1.39 mg/ml) of δ ‐endotoxin was observed at 72 h in LB . Among the sugars (glucose, sucrose, maltose or lactose) tested as additional carbon source, glucose (8 g/l) enhanced (1.82 mg/ml) the production of δ ‐endotoxin by 30%. The lethal concentration ( LC 50 ) required to kill 50% mites was estimated as 8.024 mg/ml. The δ ‐endotoxin produced by B. thuringiensis BPU 5 is shown to efficiently combat T. macfarlanei, a devastating mite infesting agricultural fields.