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A multiple PCR ‐ LDR assay for identification of two invasive cryptic species of whiteflies Bemisia tabaci
Author(s) -
Wang H.,
Guo J.,
Qi L.,
Li K.
Publication year - 2015
Publication title -
journal of applied entomology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 60
eISSN - 1439-0418
pISSN - 0931-2048
DOI - 10.1111/jen.12226
Subject(s) - biology , species complex , genotyping , polymerase chain reaction , cytochrome b , genetics , microbiology and biotechnology , gene , genotype , mitochondrial dna , phylogenetic tree
Abstract The MEAM 1 and MED species of the cryptic species complex Bemisia tabaci are important invasive pests that cause tremendous crop losses worldwide. A rapid and highly reliable molecular technique is necessary to identify these species because they are morphologically indistinguishable. Therefore, a multiple polymerase chain reaction coupled with a ligase detection reaction ( PCR ‐ LDR ) that was based on polymorphisms in the mitochondrial cytochrome oxidase I (mt COI ) gene of B. tabaci was developed to distinguish the two cryptic species. An assessment of the method indicated that PCR ‐ LDR provided high specificity and sensitivity in discriminating MEAM 1 ( SHB ) and MED ( SHQ ) whiteflies. In field tests, PCR ‐ LDR genotyping was performed in one 96‐well plate to identify 93 individuals collected from 8 districts in the suburbs of Shanghai. Complete concordance was observed between PCR ‐ LDR and sequencing methods. The method was used to confirm that MEAM 1 and MED were found in two districts, but only the MED was found in the other six districts. PCR ‐ LDR , which is a transplantable platform, provides an alternative method for species identification of B. tabaci at low cost.