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Geographical variation of P lutella xylostella ( L epidoptera: P lutellidae) populations revealed by mitochondrial COI gene in C hina
Author(s) -
Niu Y.Q.,
Nansen C.,
Li X.W.,
Liu T.X.
Publication year - 2014
Publication title -
journal of applied entomology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 60
eISSN - 1439-0418
pISSN - 0931-2048
DOI - 10.1111/jen.12130
Subject(s) - biology , gene flow , haplotype , genetic diversity , nucleotide diversity , mitochondrial dna , population , genetic variation , pest analysis , genetics , gene , botany , zoology , allele , demography , sociology
The diamondback moth, P lutella xylostella ( L .) ( L epidoptera: P lutellidae), is one of the most serious pests for cruciferous vegetable growers worldwide. To determine the relations of local and regional populations, we performed a mitochondrial COI gene analysis of eight P . xylostella populations from different locations in or around the Q inling M ountains and two other populations, one from B eijing and the other from G uangdong. The mt DNA divergences among the 10 populations were high, and 32 haplotypes were detected in 149 adults. The mean haplotype divergence was 1.7% (range 0.04–4.1%). Haplotype diversity in the 10 populations varied from 0.571 ( AK ) to 0.885 ( HZ ), and the nucleotide diversity varied from 0.00286 ( AK ) to 0.0117 ( HZ ). The results also did not show significant correlation between genetic and geographical distance. Also, the effective number of migrants between populations ( N m ) ranged from 1.43 to infinite, suggesting that population exchange and gene flow among the P . xylostella populations occurred. However, principal component analysis ( PCA ) showed that the TB and TC populations were differentiated from other populations, indicating possible across‐mountain barrier to migration and gene flow.