Premium
Susceptibility to C ry proteins of a S panish O strinia nubilalis glasshouse population repeatedly sprayed with B acillus thuringiensis formulations
Author(s) -
Crava C. M.,
Bel Y.,
Ferré J.,
Escriche B.
Publication year - 2014
Publication title -
journal of applied entomology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.795
H-Index - 60
eISSN - 1439-0418
pISSN - 0931-2048
DOI - 10.1111/jen.12070
Subject(s) - biology , ostrinia , pyralidae , population , pest analysis , horticulture , european corn borer , botany , demography , sociology
Ostrinia nubilalis H übner ( L epidoptera: C rambidae), a major pest of corn in temperate climates, can feed on other crops due to its polyphagous behaviour. In particular, this species became a serious problem in some sweet pepper commercial glasshouses in south‐eastern S pain repeatedly sprayed with B acillus thuringiensis ( B t) products to control S podoptera exigua H übner ( L epidoptera: N octuidae). The susceptibility of an O . nubilalis colony established from individuals collected in these B t‐sprayed glasshouses was compared with a reference laboratory colony. Differences in susceptibility between the two colonies to C ry1Aa, C ry1Ab, C ry1Ac and C ry2Aa proteins were found. However, our results indicate that the O . nubilalis control failure in the glasshouse was not due to selection for resistance. Intraspecific variation probably accounts for differences between the glasshouse‐derived population and the laboratory strain. This conclusion is based on several lines of evidence: the glasshouse‐derived population retained its susceptibility to a B t standard product and to most of its individual components (both in the form of protoxins and in the form of activated toxins), and it did not respond to laboratory selection with high doses of C ry1Ab.