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Hyaluronic acid biomaterial for human tissue‐engineered skin substitutes: Preclinical comparative in vivo study of wound healing
Author(s) -
SierraSánchez Á.,
FernándezGonzález A.,
LizanaMoreno A.,
EspinosaIbáñez O.,
MartinezLopez A.,
GuerreroCalvo J.,
FernándezPorcel N.,
RuizGarcía A.,
OrdóñezLuque A.,
Carriel V.,
AriasSantiago S.
Publication year - 2020
Publication title -
journal of the european academy of dermatology and venereology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.655
H-Index - 107
eISSN - 1468-3083
pISSN - 0926-9959
DOI - 10.1111/jdv.16342
Subject(s) - biomaterial , hyaluronic acid , wound healing , fibrin , medicine , in vivo , human skin , skin grafting , pathology , biomedical engineering , surgery , anatomy , immunology , biology , genetics , microbiology and biotechnology
Background There is not an ideal biomaterial for tissue‐engineered skin substitutes (TESSs), and most of the studies or existing therapies use xenogeneic origin natural biomaterials or biosynthetic scaffolds. Objective To analyse clinical, histological integration and homeostasis parameters of a human TESS manufactured with fibrin–hyaluronic acid biomaterial (HA‐Skin), grafted in immunodeficient mice for 8 weeks, and compared with the gold standard treatment (Autograft), a human TESS manufactured with fibrin–agarose biomaterial (AG‐Skin) and secondary wound healing dressings. Methods Human TESSs and autografts were implanted into BALB/c mice after surgical excision. Secondary wound healing approach was achieved with biosynthetic collagen wound dressing (Biobrane ® ) and fibrin–hyaluronic acid or fibrin–agarose biomaterial without cells (Total N = 44). Clinical integration and homeostasis parameters were evaluated every two weeks for two months. Histological and immunohistochemical analyses were performed four and eight weeks after grafting. Results HA‐Skin, AG‐Skin and Autograft groups showed a proper clinical integration and epithelization eight weeks later. Scar evaluation revealed better results for Autograft and HA‐Skin. Homeostasis analysis indicated similar values of transepidermal water loss and elasticity between HA‐Skin (6.42 ± 0.75 g/h/m 2 , 0.42 ± 0.08 AU), Autograft (6.91 ± 1.28 g/h/m 2 , 0.40 ± 0.08 AU) and healthy mouse skin (6.40 ± 0.43 g/h/m 2 , 0.35 ± 0.03 AU). Histological results showed that human TESSs and autografts presented better skin structuration and higher expression of cytokeratins. Conclusions This study suggests that human TESS based on fibrin–hyaluronic acid biomaterial could be suitable for clinical application in the treatment of several dermatological pathologies (wound healing).