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Circulating micro RNA s in extracellular vesicles as potential biomarkers for psoriatic arthritis in patients with psoriasis
Author(s) -
Pasquali L.,
Svedbom A.,
Srivastava A.,
Rosén E.,
Lindqvist U.,
Ståhle M.,
Pivarcsi A.,
Sonkoly E.
Publication year - 2020
Publication title -
journal of the european academy of dermatology and venereology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.655
H-Index - 107
eISSN - 1468-3083
pISSN - 0926-9959
DOI - 10.1111/jdv.16203
Subject(s) - psoriasis , medicine , rna , psoriatic arthritis , cohort , biomarker , gastroenterology , immunology , gene , biology , biochemistry
Background Psoriatic arthritis (PsA) develops in ~30% of patients with psoriasis. The diagnosis of PsA is challenging, and there are no reliable molecular markers in clinical use. Micro RNA s are short non‐coding regulatory RNA s, which can be actively packaged into extracellular vesicles ( EV s) and secreted to the circulation. Objectives To explore whether plasma‐derived EV micro RNA s may serve as biomarkers for PsA in patients with psoriasis. Methods Plasma samples were obtained from patients with cutaneous‐only psoriasis (PsC) and patients with psoriasis and PsA. Plasma EV s were isolated using mi RCURY ™ Exosome Isolation Kit. RNA sequencing was used to identify differentially expressed EV mi RNA s in the discovery phase (PsC, n = 15; PsA, n = 14). In the validation phase (PsC, n = 29; PsA, n = 28), 41 selected mi RNA s were analysed in plasma EV s by qPCR . The association of the identified mi RNA s with PsA was assessed by logistic regression analysis. Results RNA sequencing identified 19 plasma EV mi RNA s with significantly different levels between PsA and PsC in the discovery cohort. Significantly lower levels of plasma EV let‐7b‐5p and miR‐30e‐5p in PsA vs. PsC were confirmed in the validation cohort, and their decreased levels were found to be associated with the presence of PsA. ROC analysis revealed an AUC of 0.68 (95% CI 0.53–0.83) for let‐7b‐5p and 0.69 (95% CI 0.55–0.84) for miR‐30e‐5p. Conclusions Circulating EV micro RNA levels are altered in patients with PsA as compared with PsC. Findings of this exploratory study suggest that circulating EV micro RNA s may serve as biomarkers for arthritis in psoriasis patients.