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Validation of the BIOCHIP test for the diagnosis of bullous pemphigoid, pemphigus vulgaris and pemphigus foliaceus
Author(s) -
Yang A.,
Xuan R.,
Melbourne W.,
Tran K.,
Murrell D.F.
Publication year - 2020
Publication title -
journal of the european academy of dermatology and venereology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.655
H-Index - 107
eISSN - 1468-3083
pISSN - 0926-9959
DOI - 10.1111/jdv.15770
Subject(s) - biochip , pemphigus vulgaris , bullous pemphigoid , multiplex , pemphigus , pemphigus foliaceus , medicine , immunofluorescence , pemphigoid , iif , autoantibody , direct fluorescent antibody , paraneoplastic pemphigus , antibody , dermatology , immunology , biology , bioinformatics
Background The BIOCHIP is a novel multiplex indirect immunofluorescence technique used in the serological diagnosis of bullous pemphigoid and pemphigus. The BIOCHIP method combines the screening of autoantibodies and target antigen‐specific substrates in a single miniature incubation field. Objective To evaluate the diagnostic accuracy of the new immunofluorescence BIOCHIP multiplex tool in pemphigus and bullous pemphigoid. Methods For the validation of the BIOCHIP , sera from patients with BP ( n = 38), PF ( n = 8) and pemphigus vulgaris ( PV ) ( n = 23) were used. In addition, sera from disease control patients ( n = 63) and healthy volunteers ( n = 39) were used. The multiplex BIOCHIP and direct immunofluorescence ( DIF ) were performed for all BP , PF and PV patients. Additional indirect immunofluorescence (IIF) was performed on patients with BP , and ELISA was performed on patients with pemphigus. Results The BIOCHIP mosaic showed a sensitivity of 86.8% and specificity of 85% for BP 180 or BP 230 being positive in BP . It demonstrated a sensitivity of 75% and specificity of 97.7% for Dsg1 in PF . The BIOCHIP was found to have a sensitivity of 60.9% and specificity of 73.6% for Dsg3 in PV . Conclusion The BIOCHIP mosaic‐based immunofluorescence test is potentially a simple, time and effort saving test that can aid in the diagnosis and screening of BP , PV and PF . However, there is potential for interpretation bias and a learning curve that needs to be taken into consideration.