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Comparing acute and chronic human cutaneous leishmaniasis caused by Leishmania major and Leishmania tropica focusing on arginase activity
Author(s) -
Mortazavi H.,
Sadeghipour P.,
Taslimi Y.,
Habibzadeh S.,
Zali F.,
Zahedifard F.,
Rahmati J.,
Kamyab K.,
Ghandi N.,
Zamanian A.,
Reza Tohidinik H.,
Muller I.,
Kropf P.,
Rafati S.
Publication year - 2016
Publication title -
journal of the european academy of dermatology and venereology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.655
H-Index - 107
eISSN - 1468-3083
pISSN - 0926-9959
DOI - 10.1111/jdv.13838
Subject(s) - arginase , medicine , peripheral blood mononuclear cell , immunology , leishmania , cutaneous leishmaniasis , leishmaniasis , arginine , in vitro , biology , parasite hosting , biochemistry , amino acid , world wide web , computer science
Background Cutaneous leishmaniasis ( CL ) in Iran is mainly caused by Leishmania major ( L. major ) and L. tropica . Arginase mediated L‐arginine metabolism is an important issue in Leishmania parasite propagation. Arginase activity in human CL due to L. major and L. tropica have not been studied up to now. Objectives We aimed to compare the clinical and laboratory aspects of acute and chronic CL , focussing on arginase activity. Methods In this case–control study, 30 patients with acute CL (duration ≤ 1 year), 13 patients with chronic CL (duration ≥ 2 year) and 11 healthy controls were recruited. Arginase activity was measured in skin biopsies of lesions, peripheral blood polymorphonuclear cells ( PMN s), peripheral blood mononuclear cells ( PBMC s) and plasma by standard methods. Results The median of arginase activity in the acute lesions was higher than in chronic samples and significantly higher than in healthy controls ( P = 0.008). PMN s of both acute and chronic patients showed higher levels of arginase activity as compared to the levels in PBMC s and plasma. The median of arginase activity in the PMN s of patients with chronic CL was higher than that of patients with acute CL and significantly higher than that of the healthy controls ( P = 0.010). Conclusion The level of arginase activity in lesions of patients with acute and chronic CL was higher than the skin of healthy controls. The highest level of arginase activity was observed in PMN s from patients with chronic CL . This suggests that the high level of arginase activity in PMN s of patients with chronic CL may contribute to the chronicity.