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Impaired CD23 and CD62L expression and tissue inhibitors of metalloproteinases secretion by eosinophils in adults with atopic dermatitis
Author(s) -
Oliveira Titz T.,
Orfali R.L.,
Lollo C.,
Santos V.G.,
Silva Duarte A.J.,
Sato M.N.,
Aoki V.
Publication year - 2016
Publication title -
journal of the european academy of dermatology and venereology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.655
H-Index - 107
eISSN - 1468-3083
pISSN - 0926-9959
DOI - 10.1111/jdv.13825
Subject(s) - immunology , medicine , eosinophil , chemokine , receptor , secretion , atopic dermatitis , receptor expression , eosinophilia , cd23 , stimulation , matrix metalloproteinase , immune system , immunoglobulin e , antibody , asthma
Background Eosinophils are multifunctional, polymorphonuclear leucocytes that secrete proteins within cytoplasmic granules, such as cytokines, chemokines, metalloproteinases ( MMP s) and metalloproteinases tissue inhibitors ( TIMP s). Although eosinophilia is a hallmark of atopic dermatitis ( AD ), several functional aspects of eosinophils remain unknown. Objective We aimed to evaluate the phenotype and functional response of eosinophils under staphylococcal enterotoxin B ( SEB ) and Toll‐like receptor ( TLR )‐2/6 ( FSL ‐1) stimulation in the secretion of CCL 5, MMP s and TIMP s in adults with AD . Methods Forty‐one adult patients with AD and 45 healthy controls enrolled for the study. Phenotype of eosinophils from granulocytes of peripheral blood was analysed by flow cytometry. We performed evaluation of CCL 5 (cytometric bead array), MMP and TIMP ( ELISA ) secretion, in culture supernatants of purified eosinophils stimulated with SEB or TLR 2/6 agonist ( FSL ‐1). Results We found a higher frequency of LIN 1 − CCR 3 + eosinophils, and decreased expression of CD 23 and CD 62L receptors in eosinophils of AD patients. There was no difference in MMP and TIMP serum levels between the evaluated groups. However, we detected decreased basal levels of TIMP ‐1, TIMP ‐2 and CCL 5 in culture supernatants from purified, unstimulated eosinophils from AD patients. Conclusion In adults with AD , phenotypical features of eosinophils reveal decreased expression of early activation and L‐selectin receptors. Regarding the functional profile of purified eosinophils related to tissue remodelling in atopic dermatitis, innate immune stimulation ( TLR 2/6 agonist and SEB ) did not affect the ratio of MMP / TIMP s secretion in AD . Our findings reinforce the potential breakdown in tissue remodelling process mediated by eosinophils in AD .