Premium
Phenotypical characterization of circulating cell subsets in pyoderma gangrenosum patients: the experience of the Italian immuno‐pathology group
Author(s) -
Quaglino P.,
Fava P.,
Caproni M.,
Antiga E.,
De Simone C.,
Papini M.,
Parodi A.,
Novelli M.,
OsellaAbate S.,
Ribero S.,
Sanlorenzo M.,
Ponti R.,
Fierro M.T.,
Marzano A.V.,
Savoia P.
Publication year - 2016
Publication title -
journal of the european academy of dermatology and venereology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.655
H-Index - 107
eISSN - 1468-3083
pISSN - 0926-9959
DOI - 10.1111/jdv.13100
Subject(s) - medicine , chemokine receptor , pyoderma gangrenosum , ccr4 , flow cytometry , immunology , chemokine , phenotype , myeloid , pathology , inflammation , biology , gene , biochemistry , disease
Background No data are available as to the phenotype of circulating lymphocyte subsets in pyoderma gangrenosum ( PG ). Aim To analyse the expression of different chemokine receptors associated to T‐helper (Th)1 ( CCR 5), Th2 ( CCR 4) and Th17 ( CCR 6), as well as the regulatory T‐cell subset (Treg) and dendritic cell polarization in the blood of newly diagnosed untreated PG patients. Materials and methods Multi‐parameter flow cytometry was performed on blood samples from 10 PG patients collected at first diagnosis among centres belonging to the Italian Immuno‐pathology Group. Blood samples from 10 age‐ and sex‐matched healthy controls ( HC ) were used as controls. Results PG patients are characterized by an over‐expression in the blood of the CD 4+ CCR 5+ and CD 4+ CCR 6+ and a down‐regulation of CD 4+ CCR 4+ counts with respect to healthy subjects. Moreover, they show increased levels of myeloid derived dendritic cells type1 and reduced levels of the Treg CD 4+ CD 25high FOXP 3+ subset. Conclusions The pattern of chemokine expression argues in favour of a Th1 ( CCR 5+) and Th17 ( CCR 6+) polarization with a down‐regulation of Th2 ( CCR 4+).