Open AccessIntake of sucrose affects gut dysbiosis in patients with type 2 diabetes
Author(s) -
Hashimoto Yoshitaka,
Hamaguchi Masahide,
Kaji Ayumi,
Sakai Ryosuke,
Osaka Takafumi,
Inoue Ryo,
Kashiwagi Saori,
Mizushima Katsura,
Uchiyama Kazuhiko,
Takagi Tomohisa,
Naito Yuji,
Fukui Michiaki
Publication year - 2020
Publication title -
journal of diabetes investigation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.089
H-Index - 50
eISSN - 2040-1124
pISSN - 2040-1116
DOI - 10.1111/jdi.13293
Subject(s) - gut flora , dysbiosis , diabetes mellitus , bifidobacterium , medicine , type 2 diabetes , type 2 diabetes mellitus , bacteroidetes , feces , physiology , biology , endocrinology , microbiology and biotechnology , lactobacillus , food science , 16s ribosomal rna , bacteria , immunology , fermentation , genetics
Aims/Introduction Gut dysbiosis is generally associated with type 2 diabetes mellitus. However, the effect of habitual dietary intake on gut dysbiosis in Japanese patients with type 2 diabetes mellitus has not yet been explicated. This study investigated whether alteration of the gut microbiota was influenced by dietary intake of sucrose in Japanese patients with type 2 diabetes mellitus. Materials and Methods In this cross‐sectional study, 97 patients with type 2 diabetes mellitus and 97 healthy individuals were matched by age and sex, and then, fecal samples were obtained. Next‐generation sequencing of the 16S ribosomal ribonucleic acid gene was carried out, and functional profiles for the gut microbiota were analyzed. We selected the top 30 gut microbial genera and top 20 functional profiles for the gut microbiota specified by the weighted average difference method. The association between gut microbial genera or functional profiles and habitual dietary intake was investigated by Spearman’s rank correlation coefficient, and then, clustering analysis was carried out to clarify the impact of habitual dietary intake. Results The Actinobacteria phylum was highly abundant in patients with type 2 diabetes mellitus, whereas the Bacteroidetes phylum was less abundant. Diabetic‐type gut microbes, specifically Bacteroides and Bifidobacterium , were altered by sucrose intake at the genus level. Furthermore, sucrose intake was associated with glycolysis/gluconeogenesis in the diabetic‐type functional profiles of the gut microbiota. Conclusions The gut microbiota and functional profiles for the gut microbiota in patients with type 2 diabetes mellitus were significantly different from those in healthy individuals. Furthermore, we showed that sucrose intake was closely associated with these differences.