English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Zendy Plus

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Zendy Tools

Zendy Open

Aims/Introduction  Incretin hormone glucose‐dependent insulinotropic polypeptide/gastric inhibitory polypeptide ( GIP ) plays a key role in high‐fat diet‐induced obesity and insulin resistance.  GIP  is strongly secreted from enteroendocrine K cells by oil ingestion.  G protein‐coupled receptor (GPR) 120 and  GPR 40 are two major receptors for long chain fatty acids, and are expressed in enteroendocrine K cells. In the present study, we investigated the effect of the two receptors on oil‐induced  GIP  secretion using  GPR 120‐ and  GPR 40‐double knockout ( DKO ) mice.    Materials and Methods  Global knockout mice of  GPR 120 and  GPR 40 were crossbred to generate  DKO  mice. Oral glucose tolerance test and oral corn oil tolerance test were carried out. For analysis of the number of K cells and gene expression in K cells,  DKO  mice were crossbred with  GIP ‐green fluorescent protein knock‐in mice in which visualization and isolation of K cells can be achieved.    Results  Double knockout mice showed normal glucose‐induced  GIP  secretion, but no  GIP  secretion by oil. We then investigated the number of K cells and gene characteristics in K cells isolated from  GIP ‐green fluorescent protein knock‐in mice. Deficiency of both receptors did not affect the number of K cells in the small intestine or expression of  GIP messenger ribonucleic acid  in K cells. Furthermore, there was no significant difference in the expression of the genes associated with lipid absorption or  GIP  secretion in K cells between wild‐type and  DKO  mice.    Conclusions  Oil‐induced  GIP  secretion is triggered by the two major fatty acid receptors,  GPR 120 and  GPR 40, without changing K‐cell number or K‐cell characteristics.

journal of diabetes investigation

Free fatty acid receptors, G protein‐coupled receptor 120 and G protein‐coupled receptor 40, are essential for oil‐induced gastric inhibitory polypeptide secretion