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An analysis of genetic association in opioid dependence susceptibility
Author(s) -
Nagaya D.,
Zahari Z.,
Saleem M.,
Yahaya B. H.,
Tan S. C.,
Yusoff N. M.
Publication year - 2018
Publication title -
journal of clinical pharmacy and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.622
H-Index - 73
eISSN - 1365-2710
pISSN - 0269-4727
DOI - 10.1111/jcpt.12585
Subject(s) - single nucleotide polymorphism , snp , genetics , dynorphin , opioid , biology , novelty seeking , genotype , population , medicine , gene , psychology , receptor , opioid peptide , personality , big five personality traits , social psychology , environmental health
Summary What is known Drug addiction is a novelty‐seeking personality trait that is associated with the candidate genes OPRD 1 (opioid delta receptors), OPRK 1 (opioid kappa receptors) and PDYN (prodynorphin). However, associations between single nucleotide polymorphisms ( SNP s) rs1042114 (80G>T) of the OPRD 1 gene, rs702764 (843 A>G) of the OPRK 1 gene, and rs910080 (3′ UTR _743T>C), rs1997794 (5′ UTR ‐381A>G) and rs1022563 (3′ UTR ) of the PDYN gene and novelty seeking remain controversial as reported results have not been reproducible. Objective The goal of this study was to determine the frequencies of SNP s rs1042114, rs702764, rs1997794, rs1022563 and rs910080 in the Malaysian population and to study their association with opioid dependence in Malaysian Malays. Methods A total of 459 Malay male with opioid dependence and 543 healthy male (controls) subjects were included in this study. SNP s were genotyped using the TaqMan SNP genotyping assay. Statistical analysis was performed using Golden Helix SVS software suite to identify the distribution of allele and genotype frequencies, and SNP ‐ SNP interactions were also analysed in this study. Results and discussion SNP rs1042114 in the OPRD 1 gene is strongly associated with opiate addiction ( P =.0001). In individuals homozygous for this risk allele, the likelihood of opiate addiction is increased by a factor 1.62 (95% confidence interval ( CI ) 1.412‐1.875). Polymorphic alleles at SNP rs702764 of OPRK 1 were not associated with opioid dependence. A significant association between opioid dependence and SNP rs910080 of PDYN ( P =.0217) was detected, but there was no association for SNP s rs199774 and rs1022563. A significant interaction was also identified between homozygous wild‐type genotype TT of rs702764 with the risk genotypes TG / GG of rs1042114 (odds ratio ( OR )=2.111 (95% CI 1.227‐3.631), P =.0069) and with the risk genotypes GA / AA of rs910080 ( OR =1.415 (95% CI 1.04‐1.912), P =.0239). What is new and conclusion The results indicate that SNP s rs1042114 and rs910080 contribute to vulnerability to opioid dependence in the Malaysian Malay population. These results will help us to understand the effect of the SNP s and the SNP ‐ SNP interaction on opioid dependence and may assist in efforts to screen vulnerable individuals and match them with individually tailored prevention and treatment strategies.