Premium
A rapid method to screen poisoning causative agents in an acute care hospital in Japan
Author(s) -
Usui K.,
Mori H.,
Tachi T.,
Matsumura T.,
Mori K.,
Takeda A.,
Noguchi Y.,
Yoshimura T.,
Teramachi H.
Publication year - 2017
Publication title -
journal of clinical pharmacy and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.622
H-Index - 73
eISSN - 1365-2710
pISSN - 0269-4727
DOI - 10.1111/jcpt.12532
Subject(s) - chromatography , chemistry , nitrazepam , salicylic acid , acetaminophen , methomyl , diphenhydramine , pharmacology , medicine , diazepam , pesticide , biochemistry , agronomy , histamine , biology
Summary What is known and objective A simple, rapid analysis is required to simultaneously analyse medicinal toxicants in emergency medical care. In this regard, the analysis of blood samples by LC ‐ MS / MS equipped with a spin column, involving a rapid, simple pretreatment, has attracted attention. In this study, sample pretreatment using a Monospin C18 column was performed to screen 11 medicinal toxicants in blood samples by LC ‐ MS / MS . Methods Serum samples supplemented with 11 medical toxicants‐acetaminophen, salicylic acid, nitrazepam, diphenhydramine, bromvalerylurea, phenobarbital, amitriptyline, risperidone, fenitrothion, malathion and methomyl‐were pretreated with the Monospin C18 column according to Pretreatment I and Pretreatment II , followed by LC ‐ MS / MS analysis. Results and discussion All toxicants were not detected by a single pretreatment method but were detected by two pretreatment methods. According to Pretreatment I, 10 medicinal toxicants‐excluding salicylic acid‐were detected. The recovery rates of all medicinal toxicants, except acetaminophen and methomyl, were greater than or equal to 80%. Salicylic acid was detected by Pretreatment II , with a recovery rate of 57.1%. Although the coefficient of variation was less than that reported in previous methods employing SPE , the recovery rates were better possibly because of the simultaneous adsorption of water‐ and lipid‐soluble substances and evaporation by drying. What is new and Conclusion As LC ‐ MS / MS analysis using Monospin C18 can simultaneously and rapidly screen several medicinal toxicants present in blood samples, it is expected to be highly suitable for clinical settings.