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Differences in platelet‐rich plasma composition influence bone healing
Author(s) -
AlHamed Faez Saleh,
AbuNada Lina,
Rodan Rania,
Sarrigiannidis Stylianos,
RamirezGarcialuna Jose Luis,
Moussa Hanan,
Elkashty Osama,
Gao Qiman,
Basiri Tayebeh,
Baca Laura,
Torres Jesus,
Rancan Lisa,
Tran Simon D.,
Lordkipanidzé Marie,
Kaartinen Mari,
Badran Zahi,
Tamimi Faleh
Publication year - 2021
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/jcpe.13546
Subject(s) - platelet rich plasma , wound healing , bone healing , platelet , chemistry , medicine , immunology , anatomy
Aim Platelet‐rich plasma (PRP) is an autologous blood‐derived material that has been used to enhance bone regeneration. Clinical studies, however, reported inconsistent outcomes. This study aimed to assess the effect of changes in leucocyte and PRP (L‐PRP) composition on bone defect healing. Materials and Methods L‐PRPs were prepared using different centrifugation methods and their regenerative potential was assessed in an in‐vivo rat model. Bilateral critical‐size tibial bone defects were created and filled with single‐spin L‐PRP, double‐spin L‐PRP, or filtered L‐PRP. Empty defects and defects treated with collagen scaffolds served as controls. Rats were euthanized after 2 weeks, and their tibias were collected and analysed using micro‐CT and histology. Results Double‐spin L‐PRP contained higher concentrations of platelets than single‐spin L‐PRP and filtered L‐PRP. Filtration of single‐spin L‐PRP resulted in lower concentrations of minerals and metabolites. In vivo, double‐spin L‐PRP improved bone healing by significantly reducing the size of bone defects (1.08 ± 0.2 mm 3 ) compared to single‐spin L‐PRP (1.42 ± 0.27 mm 3 ) or filtered L‐PRP (1.38 ± 0.28 mm 3 ). There were fewer mast cells, lymphocytes, and macrophages in defects treated with double‐spin L‐PRP than in those treated with single‐spin or filtered L‐PRP. Conclusion The preparation method of L‐PRP affects their composition and potential to regenerate bone.