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TLR3 ligation affects differentiation and stemness properties of gingival mesenchymal stem/progenitor cells
Author(s) -
Mekhemar Mohamed,
Tölle Johannes,
Dörfer Christof,
Fawzy ElSayed Karim
Publication year - 2020
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/jcpe.13323
Subject(s) - mesenchymal stem cell , progenitor cell , stem cell , microbiology and biotechnology , progenitor , cellular differentiation , biology , immunology , biochemistry , gene
Aim Toll‐like receptors are key players in mesenchymal stem/progenitor cells’ micro‐environmental crosstalk, endorsing various biological reactions. For the first time, this study investigates the effects of TLR3‐ligation on gingival mesenchymal stem/progenitor cells (G‐MSCs) stemness and differentiation properties. Material and Methods G‐MSCs ( n = 5) were isolated, sorted using anti‐STRO‐1 antibodies,and sowed on culture dishes to generate colony‐forming units (CFUs), and their stem/progenitor cells’ features and TLR3 expression were characterized. Subsequently, TLR3 activation of G‐MSCs via Poly (I:C) was done, followed by an analysis of the expression of pluripotency‐related factors, mesenchymal stemness‐associated surface markers, and the ability to form CFUs and multilineage differentiation, using qualitative and quantitative histochemistry and RT‐PCR. Results G‐MSCs demonstrated all predefined stem/progenitor cells’ characteristics and TLR3 expression. TLR3‐activated G‐MSCs showed a significantly reduced ability to form CFUs and pluripotency transcriptional factors expression. Mesenchymal stem/progenitor cell‐associated surface markers and multilinear differentiation potential were significantly higher following TLR3 ligation ( p < .05, Wilcoxon signed rank test). Conclusions TLR3‐mediated activation maintains the mesenchymal stem/progenitor cells phenotype and drives G‐MSCs’ differentiation and commitment, with a shift away from an undifferentiated pluripotent cellular phenotype. This distinctive modulation could influence potential therapeutic applications of G‐MSCs.