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Interplay between vitamin D receptor Fok I polymorphism and smoking influences Porphyromonas gingivalis proportions in subgingival plaque
Author(s) -
Torrungruang Kitti,
Chantarangsu Soranun,
Sura Thanyachai,
Thienpramuk Lalitsara
Publication year - 2020
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/jcpe.13307
Subject(s) - foki , porphyromonas gingivalis , periodontitis , chronic periodontitis , genotype , calcitriol receptor , medicine , genotyping , clinical attachment loss , aggressive periodontitis , bleeding on probing , vitamin d and neurology , polymorphism (computer science) , gastroenterology , biology , genetics , gene
Aim This cross‐sectional study investigated the effect of the vitamin D receptor (VDR) Fok I polymorphism and its interactions with smoking/drinking on the proportions of periodontal pathogens and periodontitis severity. Materials and Methods Fok I genotyping and bacterial quantification were performed using real‐time polymerase chain reaction. Periodontitis severity was determined using mean clinical attachment level (CAL). Regression analyses examined the associations between the Fok I polymorphism (rs2228570) and bacterial proportions or periodontitis severity. Effect modification by smoking or drinking was assessed. Results The study population comprised 1,460 individuals, aged 39–66 years. After multivariable adjustment, the Fok I risk genotypes (CC + CT) were associated with elevated Porphyromonas gingivalis proportions (regression coefficient (β) =0.294 ± 0.139; p = .034) and increased mean CAL (β = 0.130 ± 0.048; p = .007). The effect of the Fok I polymorphism on P . gingivalis proportions was greater in smokers (β = 0.897 ± 0.328; p = .006) compared to non‐smokers (β = 0.164 ± 0.153; p = .282) and in drinkers (β = 0.668 ± 0.242; p = .006) compared to non‐drinkers (β = 0.114 ± 0.169; p = .500). The genotype*smoking interaction for P . gingivalis proportions was significant ( p = .043), whereas the genotype*drinking interaction was not ( p = .061). Similar results were found for the effect of the genotype*smoking/drinking interaction on mean CAL. Conclusions These findings suggest that the interplay between the host genotype and smoking is important in determining the subgingival microbial composition and periodontitis severity.