Premium
Periodontal tissue regeneration after low‐intensity pulsed ultrasound stimulation with or without intra‐marrow perforation in two‐wall intra‐bony defects—A pilot study in dogs
Author(s) -
Shirakata Yoshinori,
Imafuji Takatomo,
Sena Kotaro,
Shinohara Yukiya,
Nakamura Toshiaki,
Noguchi Kazuyuki
Publication year - 2020
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/jcpe.13197
Subject(s) - low intensity pulsed ultrasound , beagle , medicine , perforation , cementum , bone healing , regeneration (biology) , dentistry , ultrasound , anatomy , therapeutic ultrasound , biology , radiology , materials science , dentin , metallurgy , punching , microbiology and biotechnology
Aim To evaluate the effects of low‐intensity pulsed ultrasound (LIPUS) with/without intra‐marrow perforation (IMP) on periodontal healing in two‐wall intra‐bony defects in dogs. Materials and Methods Two‐wall intra‐bony defects (5 mm wide, 5 mm deep) were created at the distal and mesial aspects of mandibular premolars in four beagle dogs (four defects per dog). The 16 defects were divided into four treatment groups: IMP, LIPUS, IMP + LIPUS (IMP/LIPUS) and control (open flap debridement). The LIPUS and IMP/LIPUS sites received daily LIPUS exposure for 3 weeks starting 1 week after surgery. The animals were euthanized 4 weeks after surgery for histologic evaluation. Results There was significantly greater new bone formation at LIPUS (2.93 ± 0.74 mm) and IMP/LIPUS (3.18 ± 0.52 mm) sites than at control sites (1.65 ± 0.46 mm). New bone area at LIPUS (6.36 ± 2.28 mm 2 ) and IMP/LIPUS (6.13 ± 1.25 mm 2 ) sites was significantly greater than that at control sites (2.15 ± 1.75 mm 2 ). New cementum length at LIPUS sites (4.09 ± 0.75 mm) was significantly greater than that at control (2.29 ± 1.02 mm) and IMP (2.41 ± 0.41 mm) sites. No significant difference was observed between LIPUS and IMP/LIPUS sites in any histomorphometric parameter. Conclusions These findings suggest that LIPUS effectively promotes periodontal regeneration in two‐wall intra‐bony defects in dogs.