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Dysregulation of human miRNAs and increased prevalence of HHV miRNAs in obese periodontitis subjects
Author(s) -
Naqvi Afsar R.,
Brambila Maria F.,
Martínez Gloria,
Chapa Gabriela,
Nares Salvador
Publication year - 2019
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/jcpe.13040
Subject(s) - rna , transcriptome , microrna , gene expression , inflammation , periodontitis , gene , biology , real time polymerase chain reaction , rna extraction , immunology , medicine , genetics
Aim To evaluate human and herpesvirus‐encoded micro RNA (mi RNA ) expression in healthy and diseased gingiva of obese and non‐obese subjects and compare the impact of localized and systemic inflammation on human mi RNA profiles. Material and methods Healthy and inflamed gingival biopsies were collected from obese and non‐obese subjects. Human and herpesvirus mi RNA expression was quantified using quantitative PCR . Predicted targets of dysregulated mi RNA s were identified using bioinformatics analysis, validated by dual luciferase assays and their expression assessed in healthy and diseased tissues. Results Our results show differential expression of mi RNA s in both diseased groups compared to healthy counterparts. MMP ‐16 is identified as a novel target of mi RNA s altered in disease. Expression analysis of genes predicted as target of differentially expressed mi RNA s show significant changes in disease compared with healthy tissues. Finally, quantitation of four herpesvirus‐derived viral mi RNA s show that the expression and prevalence of herpesvirus mi RNA s in diseased gingiva of obese subjects. Conclusion Our findings show that mi RNA (both cellular and virus) expression is differentially responsive to local and systemic inflammation. Some of these mi RNA s can modulate key cellular genes with direct consequences on inflammatory pathways suggesting their impact on oral tissue transcriptome and functions.