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Nupharidine enhances Aggregatibacter actinomycetemcomitans clearance by priming neutrophils and augmenting their effector functions
Author(s) -
Levy Dan Henry,
Chapple Iain L. C.,
Shapira Lior,
GolanGoldhirsh Avi,
Gopas Jacob,
Polak David
Publication year - 2019
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/jcpe.13036
Subject(s) - aggregatibacter actinomycetemcomitans , phagocytosis , microbiology and biotechnology , neutrophil extracellular traps , periodontitis , biology , immunology , immune system , respiratory burst , priming (agriculture) , granulocyte , inflammation , medicine , porphyromonas gingivalis , botany , germination
Objectives Nupharidine (6,6′‐Dihydroxythiobinupharidine), purified from the aquatic plant Nuphar lutea leaves (Water lily) prompts antimicrobial activity of immune cells. The aim of the study was to test the effect of Nupharidine on neutrophil function against Aggregatibacter actinomycetemcomitans , JP 2 clone (Aa‐ JP 2). Methods Neutrophils derived from the human cell line HL 60 and human peripheral blood derived from aggressive periodontitis and periodontally healthy subjects were incubated with Nupharidine or vehicle and inoculated with JP 2. Bacterial survival was tested using viable counts on blood agar ( CFU 's). Neutrophils’ necrosis/apoptosis, reactive oxygen species ( ROS ) production, phagocytosis and neutrophil extracellular traps ( NET ) production following infection were tested, as well as markers of neutrophil priming. Results Nupharidine had no direct bactericidal effect on JP 2, but it enhanced Aa‐ JP 2 clearance by neutrophils. Nupharidine enhanced neutrophil phagocytosis, ROS production and NET formation during JP 2 infection. Furthermore, Nupharidine enhanced the expression of certain markers of neutrophils priming, specifically iCAM 1, DECTIN ‐2 and intracellular IL ‐1β. Conclusion Nupharidine was shown to promote neutrophil effector bactericidal functions, boosting Aa‐ JP 2 clearance. The results point to the potential of Nupharidine as an adjunctive agent in the treatment of Aa‐ JP 2 periodontitis, but this should be tested initially using pre‐clinical and clinical studies.