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Healing kinetics of oral soft tissue wounds treated with recombinant epidermal growth factor: Translation from a canine model
Author(s) -
Ben Amara Heithem,
Thoma Daniel S.,
Schwarz Frank,
Song Hyun Young,
Capetillo Joseph,
Koo KiTae
Publication year - 2019
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/jcpe.13035
Subject(s) - epidermal growth factor , wound healing , granulation tissue , medicine , soft tissue , recombinant dna , pathology , surgery , chemistry , receptor , biochemistry , gene
Objective To test whether or not topically administered recombinant human epidermal growth factor (rh EGF ) accelerates the early healing phase of oral soft tissue wounds. Methods One day following the creation of palatal defects ( n = 6/animal), 14 dogs were allocated to one of the following five groups: spontaneous healing ( SH ), vehicle ointment (V), vehicle ointment + rh EGF at concentrations of 1 μg/g ( EGF 1), 10 μg/g ( EGF 10) or 50 μg/g ( EGF 50). Topical administration of ointments was repeated twice per day until sacrifice at days 8 and 16. Wound area was clinically monitored. Keratinocytes proliferation (Ki67‐immunolabelling), inflammatory response ( IR ) and areas of collagen (C) and granulation tissue ( GT ) were histologically measured. Kruskal–Wallis test with Dunnett correction was used for multiple group statistical comparisons. Results Clinically, in comparison with SH , a significantly smaller wound area was observed in groups EGF 1 and EGF 10 at day 8 ( p < 0.05). At day 16, wound closure reached 97.8% in group EGF 1 compared to 83.2% in group SH , albeit no statistically different. Histologically, at day 8, significantly more GT was observed in group EGF 10 compared to all other groups ( p < 0.05). At day 16, in addition to a higher Ki67‐immunolabelling, groups EGF 1 and EGF 10 demonstrated a significant decrease in GT and IR with more deposition of C compared to the other groups ( p < 0.05). Conclusion Application of rh EGF enhanced the early healing of acute oral soft tissue wounds compared to SH , predominantly at concentrations of 1 and 10 μg/g.