Premium
Glycaemic status affects the subgingival microbiome of diabetic patients
Author(s) -
Longo Priscila L.,
Dabdoub Shareef,
Kumar Purnima,
Artese Hilana P. C.,
Dib Sergio A.,
Romito Giuseppe A.,
Mayer Marcia Pinto Alves
Publication year - 2018
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/jcpe.12908
Subject(s) - periodontitis , microbiome , medicine , gingival and periodontal pocket , chronic periodontitis , microbiology and biotechnology , diabetes mellitus , capnocytophaga , dentistry , biology , bioinformatics , bacteria , endocrinology , genetics
Aim Periodontitis is correlated with type 2 diabetes mellitus (T2 DM ), but little is known about glycaemic status effect on subgingival microbiota associated with periodontitis. This study evaluated if periodontal microbiome of T2 DM patients is affected by glycaemic status. Materials and methods Twenty‐one T2 DM non‐smoking patients with chronic periodontitis and body mass index ≤40 kg/m 2 were allocated into two groups according to systemic glycaemic status: inadequate ( DMI ‐ HbA1c ≥ 8%) and adequate ( DMA ‐ HbA1c <7.8%). Subgingival biofilm was collected from sites with moderate ( PD = 4–6 mm) and severe disease ( PD ≥ 7 mm) in two quadrants. The V5–V6 hypervariable region of the 16Sr RNA was sequenced using the GS ‐ FLX ‐454 Titanium platform. Sequences were compared with HOMD database using QIIME and PhyloTo AST pipelines. Statistical comparisons were made using two‐sample t ‐tests. Results DMA microbiome presented higher diversity than DMI . Inadequate glycaemic control favoured fermenting species, especially those associated with propionate/succinate production, whereas those forming butyrate/pyruvate was decreased in DMI . Higher abundances of anginosus group and Streptococcus agalactiae in DMI may indicate that subgingival sites can be reservoir of potentially invasive pathogens. Altered subgingival microbiome in DMI may represent an additional challenge in the periodontal treatment of these patients and in the prevention of more invasive infections. Conclusion Glycaemic status in T2 DM patients seems to modulate subgingival biofilm composition.