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Micro RNA ‐146a and micro RNA ‐155 as novel crevicular fluid biomarkers for periodontitis in non‐diabetic and type 2 diabetic patients
Author(s) -
Radović Nikola,
Nikolić Jakoba Nataša,
Petrović Nina,
Milosavljević Aleksandra,
Brković Božidar,
Roganović Jelena
Publication year - 2018
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/jcpe.12888
Subject(s) - periodontitis , diabetes mellitus , medicine , downregulation and upregulation , type 2 diabetes , type 2 diabetes mellitus , microrna , superoxide dismutase , gastroenterology , real time polymerase chain reaction , biomarker , chronic periodontitis , endocrinology , chemistry , oxidative stress , biochemistry , gene
Abstract Aim Recent studies point at the crucial role of epigenetic mechanisms in the development of multifactorial diseases such as periodontitis and diabetes mellitus ( DM ) type 2. In addition, circulatory micro RNA s (miRs) have emerged as novel biomarkers for various diseases. Aim of this study was to investigate the levels of miR‐146a and miR‐155 and superoxide dismutase ( SOD ) activity in gingival crevicular fluid ( GCF ) of periodontitis patients with ( CPDM ) and without ( CP ) DM type 2 as well as in periodontally healthy, control groups ( PHDM and PH , respectively). Material and methods miR modulation was analysed using quantitative real‐time PCR while SOD activity was measured spectrophotometrically. Results The upregulation of miR‐146a and miR‐155 was observed in CP and CPDM patients’ baseline, while the levels decreased after 6 weeks of the non‐surgical therapy to the levels comparable to PH and PHDM , respectively. Expression levels of miRs positively correlated with SOD activity. Levels of miR‐146a were higher in PHDM compared to PH patients. Multivariate analysis revealed that levels of miR‐146a and miR‐155 were significantly associated with periodontitis when adjusting for age and gender. Conclusions miR‐146a and miR‐155 may be considered as possible novel biomarkers for periodontitis in non‐diabetic and type 2 diabetic patients.