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Do different probing depths exhibit striking differences in microbial profiles?
Author(s) -
PérezChaparro Paula Juliana,
McCulloch John Anthony,
Mamizuka Elsa Masae,
Moraes Aline da Costa Lima,
Faveri Marcelo,
Figueiredo Luciene Cristina,
Duarte Poliana Mendes,
Feres Magda
Publication year - 2018
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/jcpe.12811
Subject(s) - bacteroidetes , microbiology and biotechnology , spirochaete , biology , periodontitis , 16s ribosomal rna , bacteroides , phylum , porphyromonas gingivalis , eubacterium , periodontal disease , fusobacterium nucleatum , bacteroidaceae , zoology , bacteria , medicine , genetics , dentistry
Aim To perform a thorough characterization of the subgingival microbiota of shallow, moderate and deep sites in subjects with chronic periodontitis (ChP). Material and methods Subgingival samples were collected from subjects with ChP ( n = 3/category of probing depth: ≤3, 4–6 and ≥7 mm) and periodontal health ( PH ). Individual samples were submitted to 16S rDNA high‐ throughput sequencing and the analysis was made using mothur and R packages. Results Nine subjects with ChP and seven with PH were included and 101 samples were evaluated. Thirteen phyla, 118 genera and 211 OTU s were detected. Taxa from Chloroflexi and Spirochaetes phyla were associated with initial stages of disease. Fretibacterium, Eubacterium[ XI ][G‐6], Desulfobulbus, Peptostreptococcaceae[ XI ][G‐1] and [G‐3], Bacteroidetes[G‐3], Bacteroidaceae[G‐1] genera and Filifactor alocis , Fretibacterium fastidiosum , Johnsonella sp HOT 166, Peptostreptococcaceae[ XIII ][G‐1] HOT 113, Porphyromonas endodontalis and Treponema sp . HOT 258, which are not conventionally associated with disease, increased with the deepening of the pockets and/or were elevated in ChP; while Streptococcus , Corynebacterium and Bergeyella genera were associated with PH ( p < .05). Conclusion Striking differences were observed between the microbiota of shallow and moderate/deep sites, but not between moderate and deep sites in ChP subjects. Differences between shallow sites in PH and ChP were also observed. The characterized microbiota included known oral microorganisms and newly identified periodontal taxa, some of them not‐yet‐cultured.