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Exploring the microbiome of healthy and diseased peri‐implant sites using Illumina sequencing
Author(s) -
SanzMartin Ignacio,
DoolittleHall Janet,
Teles Ricardo P.,
Patel Michele,
Belibasakis Georgios N.,
Hämmerle Christoph H. F.,
Jung Ronald E.,
Teles Flavia R. F.
Publication year - 2017
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/jcpe.12788
Subject(s) - microbiome , peri implantitis , bacteroidetes , biology , fusobacterium , porphyromonas gingivalis , metagenomics , illumina dye sequencing , microbiology and biotechnology , implant , 16s ribosomal rna , dna sequencing , medicine , bioinformatics , genetics , bacteroides , bacteria , gene , surgery
Aim To compare the microbiome of healthy (H) and diseased (P) peri‐implant sites and determine the core peri‐implant microbiome. Materials and Methods Submucosal biofilms from 32 H and 35 P sites were analysed using 16S rRNA sequencing (MiSeq, Illumina), QIIME and HOMI NGS . Differences between groups were determined using principal coordinate analysis ( PC oA), t tests and Wilcoxon rank sum test and FDR ‐adjusted. The peri‐implant core microbiome was determined. Results PC oA showed partitioning between H and P at all taxonomic levels. Bacteroidetes , Spirochetes and Synergistetes were higher in P, while Actinobacteria prevailed in H ( p < .05). Porphyromonas and Treponema were more abundant in P while Rothia and Neisseria were higher in H ( p < .05). The core peri‐implant microbiome contained Fusobacterium , Parvimonas and Campylobacter sp. T. denticola , and P. gingivalis levels were higher in P, as well as F. alocis , F. fastidiosum and T. maltophilum ( p < .05). Conclusion The peri‐implantitis microbiome is commensal‐depleted and pathogen‐enriched, harbouring traditional and new pathogens. The core peri‐implant microbiome harbours taxa from genera often associated with periodontal inflammation.