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Chronic periodontitis is associated with platelet factor 4 ( PF 4) secretion: A pilot study
Author(s) -
BrousseauNault Mathieu,
Kizhakkedathu Jayachandran N.,
Kim Hugh
Publication year - 2017
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/jcpe.12771
Subject(s) - gingivitis , periodontitis , periodontium , platelet , platelet factor 4 , porphyromonas gingivalis , chronic periodontitis , medicine , platelet activation , inflammation , immunology , dentistry
Aim Platelets contribute to chronic inflammation, but their role in periodontitis is not well understood. The aim of this study was to compare platelet recruitment and activation in healthy and inflamed periodontium. Materials and Methods Gingival crevicular fluid ( GCF ) samples were obtained from sites of healthy periodontium, gingivitis and periodontitis. Platelets were quantified in the GCF by staining and microscopy. GCF concentrations of platelet factor 4 ( PF 4) [ PF 4] GCF and glycoprotein II b III a ([ GPII b III a] GCF ) were determined by ELISA . Blood samples were obtained from the three patient groups. Platelets were isolated from whole blood and stimulated with lipopolysaccharide ( LPS ) from Porphyromonas gingivalis to evaluate and compare the LPS ‐induced PF 4 release. Results Compared to controls, platelet recruitment was increased at gingivitis and periodontitis sites, based on platelet counts and [ GPII b III a] GCF . [ PF 4] GCF was elevated in periodontal pockets but not at gingivitis or healthy sites. Circulating plasma levels of PF 4 were higher in patients with generalized severe periodontitis ( SP ), compared to patients with gingivitis or healthy periodontium. Platelets isolated from SP patients contained and released more PF 4 in response to P. gingivalis LPS than platelets from gingivitis or periodontally healthy patients. Conclusions Periodontitis is associated with increased platelet activation and PF 4 release, both locally and systemically.