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The role of 5‐lipoxygenase in Aggregatibacter actinomycetemcomitans ‐induced alveolar bone loss
Author(s) -
Madeira Mila F. M.,
QueirozJunior Celso M.,
Corrêa Jôice D.,
Werneck Sílvia M. C.,
Machado Fabiana S.,
Cunha Thiago M.,
Garlet Gustavo P.,
Teixeira Mauro M.,
Silva Tarcília A.,
Souza Daniele G.
Publication year - 2017
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/jcpe.12755
Subject(s) - osteoclast , aggregatibacter actinomycetemcomitans , dental alveolus , chemistry , bone resorption , in vivo , antagonist , medicine , endocrinology , lipopolysaccharide , receptor antagonist , pharmacology , receptor , immunology , periodontitis , biochemistry , biology , microbiology and biotechnology , porphyromonas gingivalis , dentistry
Aim Leukotrienes ( LT s) are pro‐inflammatory lipid mediators formed by the enzyme 5‐lipoxygenase (5‐ LO ). The involvement of 5‐ LO metabolites in periodontal disease ( PD ) is not well defined. This study aimed to assess the role of 5‐ LO in experimental PD induced by Aggregatibacter actinomycetemcomitans ( Aa ). Material and Methods In vivo experiments were carried out using SV 129 wild‐type ( WT ) and 5‐ LO ‐deficient ( 5lo ‐/‐ ) mice inoculated with Aa . Osteoclasts were stimulated in vitro with Aa LPS in the presence or not of selective inhibitors of the 5‐ LO pathway, or LTB 4 or platelet‐activating factor ( PAF ), as PAF has already been shown to increase osteoclast activity. Results In 5lo ‐/‐ mice, there were no loss of alveolar bone and less TRAP ‐positive osteoclasts in periodontal tissues, after Aa inoculation, despite local production of TNF ‐α and IL ‐6. The differentiation and activity of osteoclasts stimulated with Aa LPS were diminished in the presence of BLT 1 antagonist or 5‐ LO inhibitor, but not in the presence of cysteinyl leukotriene receptor antagonist. The osteoclast differentiation induced by PAF was impaired by the BLT 1 antagonism. Conclusion In conclusion, LTB 4 but not Cys LT s is important for Aa‐ induced alveolar bone loss. Overall, LTB 4 affects osteoclast differentiation and activity and is a key intermediate of PAF ‐induced osteoclastogenesis.