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Increased levels of soluble CD 163 in periodontitis patients
Author(s) -
Detzen Laurent,
Chen Steven C. Y.,
Cheng Bin,
Papapanou Panos N.,
Lalla Evanthia
Publication year - 2017
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/jcpe.12731
Subject(s) - periodontitis , saliva , chronic periodontitis , medicine , pathogenesis , biomarker , gastroenterology , population , immunology , biology , biochemistry , environmental health
Aim Soluble CD 163 ( sCD 163) has been implicated as a new biomarker in inflammatory conditions. The aim of this cross‐sectional study was to assess CD 163 levels systemically and locally in patients with chronic periodontitis. Methods sCD 163 levels were measured by ELISA in serum samples from 70 periodontitis and 70 periodontally healthy subjects, and in saliva samples in a subset of the population. Two gingival biopsies were harvested per subject from 20 periodontitis patients: one from a periodontally affected site, the other from a healthy site, and the relative expression of CD 163 mRNA was assessed by real‐time PCR. Results Serum sCD 163 was significantly higher in periodontitis patients compared to periodontally healthy subjects (720.0 ± 330.6 ng/ ml versus 510.7 ± 219.6 ng/ ml , respectively; p < .001). Similarly, sCD 163 levels in saliva were significantly increased in periodontitis compared to healthy subjects (3.01 ± 5.07 ng/ ml versus 1.98 ± 4.95 ng/ ml , respectively; p = .009). Serum and saliva sCD 163 levels showed a positive correlation (Kendall's tau .27, p = .018). Importantly, CD 163 gene expression was significantly higher in affected sites compared to unaffected sites in periodontitis patients, with a mean fold upregulation of 9.9 ( STD : 15.3, p = .010). Conclusion Our findings suggest that CD 163 may be involved in the pathogenesis of periodontitis and its link with systemic conditions.