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Mitochondrial dysfunction is involved in the aggravation of periodontitis by diabetes
Author(s) -
Sun Xiaoyu,
Mao Yixin,
Dai Panpan,
Li Xumin,
Gu Weiyan,
Wang Huining,
Wu Gang,
Ma Jianfeng,
Huang Shengbin
Publication year - 2017
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/jcpe.12711
Subject(s) - periodontitis , mitochondrial biogenesis , diabetes mellitus , periodontium , oxidative stress , mitochondrial dna , medicine , mitochondrion , dental alveolus , endocrinology , biology , dentistry , gene , microbiology and biotechnology , genetics
Aim To elucidate whether mitochondrial dysfunction contributes to aggravated periodontitis in diabetes. Materials and Methods Sixty‐four wistar rats were randomly assigned into four groups: control, periodontitis, diabetes, and diabetic periodontitis. Two weeks after induction of diabetes, periodontitis was induced by silk ligation for 2 weeks and thereafter evaluated by assessing alveolar bone loss and apoptosis of periodontium cells. Mitochondrial oxidative stress was detected by Mito SOX staining. Mitochondrial function was determined by measuring ATP production, and by assessing mitochondrial DNA copy number, activities of electron transport chain complexes, and biogenesis with real‐time PCR . Results Significantly severer bone loss, enhanced periodontium cell apoptosis, and mitochondrial oxidative stress were found in the rats with diabetic periodontitis than the others. Furthermore, diabetic rats with periodontitis presented severer mitochondrial dysfunction than lean rats with periodontitis, as reflected by compromised ATP production, decreased mitochondrial DNA copy number, reduced gene expression of electron transport chain complex I subunits, and impaired mitochondrial biogenesis ( p < 0.05). Multiple regression analysis further indicated a close correlation between these mitochondrial events and bone loss in diabetic periodontitis. Conclusions Mitochondrial dysfunction was positive correlated to aggravated periodontitis in diabetes and might represent a therapeutic target for diabetic periodontitis.