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Nutritional stimulation of commensal oral bacteria suppresses pathogens: the prebiotic concept
Author(s) -
Slomka Vera,
HernandezSanabria Emma,
Herrero Esteban Rodriguez,
Zaidel Lynette,
Bernaerts Kristel,
Boon Nico,
Quirynen Marc,
Teughels Wim
Publication year - 2017
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/jcpe.12700
Subject(s) - prebiotic , biofilm , bacteria , pathogenic bacteria , microbiology and biotechnology , biology , quorum sensing , stimulation , food science , genetics , neuroscience
Aim To identify potential oral prebiotics that selectively stimulate commensal, albeit beneficial bacteria of the resident oral microbial community while suppressing the growth of pathogenic bacteria. Material and Methods Using Phenotype MicroArrays as a high‐throughput method, the change in respiratory activity of 16 oral bacteria in response to 742 nutritional compounds was screened. Most promising prebiotic compounds were selected and applied in single species growth and biofilm formation assays, as well as dual species (beneficial‐pathogen) competition assays. Results Increased respiratory activity could not always be related to an increase in growth or biofilm formation. Six compounds were used in dual species competition assays to directly monitor if selective nutritional stimulation of the beneficial bacterium results in the suppression of the pathogenic bacterium. Two compounds, beta‐methyl‐ d ‐galactoside and N ‐acetyl‐ d ‐mannosamine, could be identified as potential oral prebiotic compounds, triggering selectively beneficial oral bacteria throughout the experiments and shifting dual species biofilm communities towards a beneficial dominating composition at in vitro level. Conclusion Our observations support the hypothesis that nutritional stimulation of beneficial bacteria by prebiotics could be used to restore the microbial balance in the oral cavity and by this promote oral health.