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Quantitative proteomic analysis of gingival crevicular fluids from deciduous and permanent teeth
Author(s) -
Moriya Yumi,
Obama Takashi,
Aiuchi Toshihiro,
Sugiyama Tomomi,
Endo Yumiko,
Koide Yoko,
Noguchi Emiko,
Ishizuka Motonori,
Inoue Mitsuko,
Itabe Hiroyuki,
Yamamoto Matsuo
Publication year - 2017
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/jcpe.12696
Subject(s) - deciduous teeth , deciduous , dentistry , permanent teeth , medicine , saliva , deciduous dentition , biology , botany
Aim Gingivitis commonly progresses to periodontitis in permanent dentition but rarely in deciduous teeth. Little is known about the biochemical differences between gingiva of deciduous and permanent teeth. Here, we compared the protein profiles of gingival crevicular fluids ( GCF ) from the gingiva of deciduous and permanent teeth. Materials and Methods Forty children with mixed dentition (Hellman's dental age IIIA ) were selected and GCF samples were collected from deciduous cuspids and central incisors in the maxilla. Pairs of GCF samples were labelled using isobaric tags to permit quantitative comparison of protein abundance in the samples using liquid chromatography‐electron spray ionization‐tandem mass spectrometry. Results Sixty‐two proteins were upregulated in deciduous teeth GCF and 54 in permanent teeth GCF . In particular, neutrophil‐derived proteins, including myeloperoxidase and lactoferrin, were repeatedly higher in deciduous teeth GCF than in permanent teeth GCF . These differences were verified using ELISA ( p < 0.01). In contrast, immunoglobulin components were upregulated in permanent teeth GCF . Conclusions Neutrophil‐related proteins were enriched in deciduous teeth GCF and immunoglobulins in permanent teeth GCF . This suggests that neutrophil accumulation plays a protective role in innate immunity against bacterial infection in gingival tissue of deciduous teeth.