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Palatal wound healing using a xenogeneic collagen matrix – histological outcomes of a randomized controlled clinical trial
Author(s) -
Thoma Daniel S.,
Hilbe Monika,
Bienz Stefan P.,
SanchoPuchades Manuel,
Hämmerle Christoph H. F.,
Jung Ronald E.
Publication year - 2016
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/jcpe.12624
Subject(s) - wound healing , medicine , granulation tissue , connective tissue , pathology , matrix (chemical analysis) , keratin , histology , surgery , chemistry , chromatography
Aim The aim of this study was to test whether or not a collagen matrix can improve early wound healing compared to spontaneous healing based on histological and immunohistologic analyses. Methods In 20 volunteers, 6 mm punch biopsies were harvested at the palate. A xenogeneic collagen matrix ( XCM ) was sutured in one site; the other one was left untreated (control). Biopsies with a diameter of 8 mm were subsequently obtained at 4, 8, 15 and 29 days and histological and immunohistologic analyses were performed. Results At day 4, wound bed keratinization amounted to 12.4 ± 7.5% (control) and 18.0 ± 10.2% ( XCM ). This increased up to day 8 (19.7 ± 25.5% control; 29.1 ± 8.0% XCM ) and reached complete keratinization at day 15 in both groups. The quantitative analyses of the superficial compartment measured an increase in the amount of granulation tissue (32–88% control; 14–41% XCM ) from day 4 to day 8. Angiogenesis was first detected at 8 days. At day 29, the amount of connective tissue in all compartments reached values similar to the native tissue at baseline. Conclusions The application of a XCM as a wound dressing on palatal wounds might be beneficial in the early stages of wound healing. Further research with a larger sample size is needed to confirm these results.