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Localized aggressive periodontitis immune response to healthy and diseased subgingival plaque
Author(s) -
Shaddox Luciana M.,
Spencer William P.,
Velsko Irina M.,
AlKassab Hiba,
Huang Hong,
Calderon Nadia,
Aukhil Ikramuddin,
Wallet Shan M.
Publication year - 2016
Publication title -
journal of clinical periodontology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.456
H-Index - 151
eISSN - 1600-051X
pISSN - 0303-6979
DOI - 10.1111/jcpe.12560
Subject(s) - periodontitis , medicine , chemokine , immune system , aggressive periodontitis , immunology
Aim The objective of this case–control study was to compare the inflammatory response of peripheral blood from localized aggressive periodontitis ( LAP ) patients when stimulated with healthy or diseased plaque samples. Materials and Methods Whole blood and subgingival plaque samples were collected from 13 LAP subjects, 14 siblings of LAP subjects and six periodontally healthy individuals. Whole blood was stimulated for 24 h with plaque samples generated from healthy or diseased sites. The levels of 14 cyto/chemokines were detected using multiplex technology. Results Localized aggressive periodontitis‐derived cultures displayed higher levels of G‐CSF , INF γ , IL 10, IL 12p40, IL 1 β , IL ‐6, IL ‐8, MCP ‐1, MIP ‐1 α , and TNF α , than control cultures regardless of stimulus used. Whole blood from healthy siblings displayed higher levels of IL ‐6 compared to control subjects, but lower levels than those observed in cultures from LAP participants. Conclusions This study suggests that although bacteria is an important factor in eliciting the hyper‐inflammatory response observed in LAP patients, the predisposition of host's response to bacterial presence may play a more significant role than the components of the stimulatory plaque.